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KMID : 1098420100180020105
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Korean Journal of Medicinal Crop Science
2010 Volume.18 No. 2 p.105 ~ p.112
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Anti-inflammatory Function of the Sophora Japonica Extract Rutin: The Inhibitory Effect of Rutin of Korean Sophora japonica on the Productions of NO and TNF-alpha form Mouse Peritoneal Macrophages
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Lee Mu-Hong
Jeong Jae-Hyun
Jeong Myeong-Soo
Chang Sung-Ho
Her Erk
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Abstract
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Korean Sophora japonica has been found to posses an anti-inflammatory activity. In this study, Korean Sophora japonica extract, rutin, was used to know whether rutin inhibits to produce inflammatory mediators NO and TNF- α from the mouse peritoneal macrophages that were treated an inflammatory agent LPS. The rutin-1 hr pretreated macrophages were incubated with LPS for 0.5~5 hrs, and then collected the supernatant and the cell lysate for measurements of the level of iNOS, NO, TNF-α mRNA, TNF-α, and p-NF-κB. Minimal and maximal effective doses of the rutin on them were 1 and 100㎍/㎖, respectively. The maximal effective dose of rutin certainly inhibted the productions of iNOS, NO, TNF-α mRNA, TNF-α, and p-NF-κB from the LPS-treated macrophages (p < 0.0001). Its ED50 for inhibition of TNF-α mRNA and p-NF-κB was 5㎍/㎖, and for iNOS, NO, and TNF-α was 10㎍/㎖. The rutin did not have any cytotoxic effect. As the results, the Sophora japonica rutin could be a good candidate for an anti-inflammatory action.
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KEYWORD
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Sophora japonica, Rutin, Anti-imflammation, Mouse Peritoneal Macrophages, TNF-α, NF-kB
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